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Binding, Stability, Kinetics |
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Isothermal Titration Calorimetry (ITC) and Binding Studies Isothermal Titration Calorimetry (ITC) is the universal detector for the study of biomolecular interactions, since heat is absorbed or generated in essentially all binding events. ITC is a true in-solution method - there is no chemical tagging or immobilization of binding components. A single ITC experiment measures the binding constant (KB), stoichiometry of binding (n), and thermodynamics of binding (DH, DG). These data are used to characterize the structure, activity, and function of proteins, nucleic acids, lipids, and other biomolecules. ITC data are also used to design and optimize drugs. Differential Scanning Calorimetry (DSC) and Protein Stability A primary goal of protein engineering, rational drug design, and biopharmaceutical formulation is the development, production, and storage of stable proteins with full functionality. It is important to know how proteins fold into their biologically active states, and how these active states are stabilized. Differential Scanning Calorimetry (DSC) is used to investigate protein stability, and is combined with other biophysical methods to link thermodynamics, structure, and function. Isothermal Titration Calorimetry (ITC) and Enzyme Kinetics Biomolecular interactions and enzyme catalysis are central to all biochemical pathways. Nearly half of all drug targets are enzymes. Isothermal Titration Calorimetry (ITC) is well-established in the study of affinity of molecular interactions, and is now becoming a mainstream tool in the study of enzyme kinetics. The strength of the technique lies in the universal nature of ITC. Traditional enzyme assays utilize a probe to monitor either substrate depletion or product formation. These probes are system-dependent and must be optimized for each reaction under specific conditions. Also, the substrate may need to be modified which could interfere with the catalysis reaction. For optical methods, the experimental conditions can affect the detection system, preventing accurate measurements. This means that with traditional assay methods, many enzymes do not have practical assays. |
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High-sensitivity DSC and isothermal microcalorimetry (-20 to 120°C) with in-situ mixing possibilities |
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µDSC3 evoµDSC3 evo is the lastest generation of the most popular microcalorimeter of Setaram. Its high sensitivity and versatility makes this instrument a star in many industrial and academic research laboratories all around the world. See more. |
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High-sensitivity DSC and microcalorimetry (-45 to 120°C) |
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µDSC7 evoEquipped with the exclusive "3D-Sensor Inside" Technology, the microcalorimeter Micro DSC VII is mainly designed for the study of substances in a confined environment (denaturation, transition, gelification, reaction, etc.) with rising and falling temperatures (no outside cooling system is needed) over a wide temperature range (–45 to 120°C). See more. |
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- November 03, 2010 - November 03, 2010
BATTERY SAFETY 2010
Boston, MA - USA
We will also be holding a workshop on November 4th entitled:
An Introduction to Assesment of Batteries & Materials Using Calorimetry and Thermal Analysis
A seminar designed to help understand the applications of calorimetry and thermal analysis in the assessment of batteries, cells and materials development. This includes everything from screening materials, to self discharge and of course abuse and overcharge testing.
The agenda is a series of presentations that introduce the techniques, principles of operation and key measurement capabilities of different techniques as well as a review of data and publications in the recent months that describe experimental procedures being applied today throughout the battery world. - October 18, 2010 - October 22, 2010
MATERIAUX 2010
Nantes, FRANCE
Stand 23
Co-organized by more twenty learned societies, these events have been meeting opportunities between the national actors in the various fields of Materials Science.
For this 3rd edition, 14 symposiums are proposed whose:
- Civil engineering and Futur Habitat
- Energy Storage & Conversion
- Materials for Transport
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- Thermodynamics and Materials for High Temperatures
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- Material stakes for the nuclear reactor (generation IV) - September 05, 2010 - September 10, 2010
SCTE 2010
Annecy, FRANCE
17th International Conference on Solid Compounds of Transition Elements
SCTE 2010 will be held in Annecy September, 5-10, 2010. The conference will be jointly organized by Institut Néel, CNRS-Université Joseph Fourier, Grenoble and Institut de Chimie de la Matière Condensée de Bordeaux, CNRS- Université Bordeaux 1, Bordeaux.
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